Coding

Part:BBa_K4275028:Design

Designed by: Haoming Qin   Group: iGEM22_GreatBay_SCIE   (2022-10-09)


eforRED-dockerin I


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. The eforRED domain and the DocI domain are interspaced with a short GS linker (GGGGS)1, to avoid the interference of DocI:CohI interaction by eforRED chromoprotein.

2. The DocI domain is situated on the C terminal of the fusion construct whilst the eforRED chromoprotein is situated at the N terminal.

3. The fusion protein is designed to prove the high-affinity of DocI:CohI interaction.

Source

Echinopora forskaliana (eforRED chromoprotein)
Clostridium thermocellum (Dockerin-I domain)

References

1. Brás, Joana L.A., et al. “Escherichia Coli Expression, Purification, Crystallization, and Structure Determination of Bacterial Cohesin–Dockerin Complexes.” Cellulases, 2012, pp. 395–415, 10.1016/b978-0-12-415931-0.00021-5.

2. Part: "Bba K592012 - Parts.Igem.Org". Parts.Igem.Org, 2022, https://parts.igem.org/Part:BBa_K592012.